Why Do Some Pigments Move Further Up the Chromatography

Usually smaller molecules will move farther than larger ones. Separation of Plant Pigments Using Chromatography.


Plant Pigments Amp Chromatography Lab

Why do pigments move up the chromatography paper.

. Appatent is added as a qualifier because often polar pigment molecules dissolved in non polar media might dimerize trimerize etc. The more soluble a pigment is the more readily it will dissolve in the mobile phase and the farther it will usually travel as it is carried by the water being absorbed into the paper. Some pigments dissolve in water easier and are pulled with the water farther up the paper.

Others are more attracted to the paper and move more slowly. The solvent carries the dissolved pigments as it moves up the paper. A pigment that is the most soluble will travel the greatest distance and a pigment that is less soluble will move a shorter distance.

Therefore the pigments move at different rates. Why do some pigments move further up the chromatography. Pigment molecule apparent size is a factor.

Subsequently question is why does carotene travel the farthest. The pigments are carried along at different rates because they are not equally soluble. The solvent carries the dissolved pigments as it moves up the paper.

What factors are involved in the separation of pigments. Some pigments dissolve in water easier and are pulled with the water farther up the paper. Also know why do some pigments travel further in chromatography.

In other words why do some pigments move further up the TLC plate than others. Be specific about each pigment. The solvent carries the dissolved pigments as it moves up the paper.

The less the polarity of the compound the greater its interaction with the mobile solvent so it moves up the TLC plate further. Carotene moves the farthest because it is the most nonpolar of the pigments and it is attracted more strongly to the acetone-ligroin mixture mobile phase than to the paper. According to the basic definition of chromatography what determines the partitioning or distribution of the pigments on the TLC plate.

This stronger nonbonded interaction with the mobile phase indicates that carotene is the most nonpolar pigment found in spinach chloroplasts. Others are more attracted to the paper and move more slowly. Also know why do some pigments travel further in chromatography.

There are always likely to be a list of affects in such seperations. Therefore the less soluble pigments will move slower up the paper than the more soluble pigments. Why do some pigments move up further up the TLC plate than others.

Also why do some pigments travel further in chromatography. Answer 1 of 3. The more soluble the pigment the farther up the pigment will move.

Others are more attracted to the paper and move more slowly. Some pigments dissolve in water easier and are pulled with the water farther up the paper. Usually smaller molecules will move farther than larger ones.

Why Do Some Pigments Move Further Up The Chromatography. The pigments are carried at different rates because they are not equally soluble.


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